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Microbial Biotechnology and Enzyme Engineering >> Presentation
Laboratory Name : Laboratory of Microbial Biotechnology and Enzyme Engineering
Acronym: LBMIE
Code:  LR15CBS06
Responsible of  Laboratory  : Prof. Samir BEJAR

The (LMBEE) was created as part of the Centre de Biotechnology of Sfax (CBS) “Contrats Programmes LMBEE-CBS 2015-2018”. The program aims to propose microorganisms and microbial enzymes of industrial interest for the economic sector. These enzymes may be screened by conventional or, alternatively, functional metagenomic methods to generate genetic information on cultivable and uncultivable microorganisms through the exploitation of metagenomic libraries. Research on the intestinal microbiota of the dromedary camel is currently underway, and research on other biotopes is forthcoming. Enzyme screening will be complemented by the analysis of biochemical and molecular properties as well as structure-function relationships so as to identify the underlying structural determinants and important amino acid residues. This will help tailor enzymes with desired properties through protein engineering to suit a wide range of industrial applications. We also plan to optimize the conditions for the incorporation of these enzymes into commercial preparations ready for use in industrial applications. The enzymes particularly targeted by this program include those  required  by the detergent, leather tanning and processing (proteases, keratinases, amylases, peroxidases), bread making (amylase, xylananses), confectionary (D-fructose, D-tagatose and palatinose), and animal feed (β-glucanases, xylanases) industries. Furthermore, and to contribute to the national efforts to combat the fire blight disease, one of the most dangerous diseases of apple pear trees caused by Erwinia amylovora, we plan to develop entophytes able to inhibit this pathogen and to screen specific bacteriophages for its biological control. Our program also seeks to express enzymes able to hydrolyze levan and its derivatives, which are responsible for the antibiotic resistance of Erwinia amylovora.